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PDB and Data Archiving Curriculum

Understanding PDB Validation: Which experimental structures should I rely on?

Webinar hosted by RCSB PDB/Rutgers Institute for Quantitative Biomedicine | May 14, 2024

Learn about primary PDB structure quality metrics, including those in the validation slider graphic; how PDB structure quality varies across the archive; and identify good structure(s) for research from RCSB.org.

After watching the videos featured in this course, you will be able to:

  • Be able to interpret the primary PDB structure quality metrics
  • Understand how PDB structure quality varies across the archive
  • Know how to identify quality structure(s) for research from RCSB.org

Additional materials for this course are available:

Click on the image below to play the video.

Protein Data Bank (PDB) and quality variation of structures

Stephen K. Burley

Director, RCSB PDB

Understanding PDB Validation: Which experimental structures should I rely on?

Chenghua Shao

Scientific Software Developer/KPI Evaluator, RCSB PDB/Rutgers


In this video:

  • 5:38
    wwPDB Validation Overview
  • 13:22
    wwPDB Validation of chemical geometry for PDB structures determined by all methods
  • 17:08
    wwPDB Validation of macromolecular crystallography (MX) structures
  • 23:27
    Validation of 3D electron microscopy (3DEM) structures
  • 29:41
    RCSB.org access to validation reports and quality review in 3D
  • 31:44
    RCSB.org structure confidence review in 3D
  • 33:53
    RCSB.org interactive ligand quality review
  • 43:47
    Q&A